Possibly used agilent zorbax rx c18 hplc column, 250 x 4. Here are some more info about the different agilent bonded phases. Hichrom chromatography columns and supplies hichrom limited 1 the markham centre, station road theale, reading, berks, rg7 4pe, uk tel. Zorbax prepht 80 stablebond columns email this page print page not yet rated request a customer account. Agilent zorbax stablebond sb cn description agilent zorbax sb cn is densely covered, sterically protected, diisopropyl cyanopropylsilane stationary phase bonded to zorbax silica. Phenomenex uhplc, hplc, spe, gc leader in analytical. Equivalent columns separation methods technologies inc. Phenomenex is a global manufacturer of uhplc, hplc columns, gc columns, sample preparation products and chromatography accessories and consumables. Regeneration of reverse c18 column agilent community.
The sb aq surface chemistry is designed to allow the alkyl phase to remain fully accessible in highly aqueous mobile phases, thereby preventing phase collapse where an alkyl chain, such as c18, collapses or mats down. Stationary phase specifications phases compared according to relative. But of course both columns might provide separation between your compounds of interest. The densely covered, sterically protected phase is chemically stable and gives longer column life, even for aggressive mobile. Zorbax columns for analytical hplc zorbax eclipse xdb.
Begin your method development with zorbax eclipse plus c18 columns for. Column characteristics a typical quality control test chromatogram for a zorbax rrhd sb c18 2. Widepore hydrophobic selectivity for nonpolar compounds. These columns provide good peak shape over a wide ph range 29 for additional method development flexibility with one family of columns. Agilent zorbax eclipse plus c18 agilent zorbax eclipse plus c8 agilent zorbax sb aq agilent zorbax eclipse plus phenyl hexyl agilent zorbax sb cn agilent zorbax sb c18 agilent zorbax bonus rp table 2. Zorbax 300sbc18 is a microparticulate column packing designed specifically for reversedphase. Oxidized insulin chain assessment using reversed phase agilent zorbax rrhd 300sb c18.
Up to twelve chemistries, depending on the particle size, including sb c18 and sb c8 for low ph applications and poroshell hph c18 and hphc8 for high ph applications easy method transfer to zorbax bonded phases and within the poroshell 120 family, for highest productivity from lab to lab, around the world. In some cases the recommended comparable columns may give slightly different selectivity, and may lead to improved and more reliable separation. Profiling capsaicinoids in spicy foods and food products. Agilent zorbax rapid resolution high definition 300sb c18 threaded column data sheet general description zorbax rapid resolution high definition rrhd 300sb c18 threaded columns are specially designed for use with ultrahigh performance liquid chromatographs uhplcs such as the agilent 1290 infinity lc and can be used up to an operating. Read through the datasheet to determine the maximum operating pressure for the column. Zorbax sb c8 pn 883975906 zorbax eclipse xdbc8 pn 993967906 competitive c8. Zorbax sbc18 is a unique microparticulate c18 pack ing used for reversedphase high performance liquid chromatography. Choose these bonded phases for the best performance and longest lifetime at low ph ph 12. Zorbax columns for analytical hplc zorbax eclipse xdb hplc columns.
The agilent zorbax eclipse xdb columns c18, c8, and phenyl are designed to provide good peak shape for basic, acidic, and neutral compounds over the ph range 29. Agilent zorbax stablebond sbc18 is made by chemically bonding a diiosobutyl noctadecylsilane c18 stationary phase to highpurity zorbax silica. Zorbax hplc columns for lcms and high throughput hplc. Agilent zorbax rapid resolution high definition 300sbc18. The densely covered, sterically protected phase is chemically stable and gives longer column life, even for aggressive mobile phases for example, ph zorbax extend c18 column specifications and options zorbax extend c18 agilent zorbax extend c18 offers high efficiency and long life at high ph up to 11. Agilent zorbax eclipse xdb columns c18, c8, phenyl and cn provide four bonded phase choices for method development optimization. Hi guys, i have to analyze a sample of sodium butyrate powder from dietary supplement in hplc without using phosphate buffer. Agilent zorbax stablebond columns use patented, unique, nonfunctional silanes with bulky diisobutyl sb c18 or diisopropyl sb c8, sb c3, sb phenyl, sb cn, and sb aq side chain groups that sterically protect the key siloxane bond to the silica surface from hydrolytic attack at low ph. View online or download agilent zorbax 300sbcn user manual. All agilent zorbax columns are uniquely optimized for specific.
Agilent technologies, one of the leading technology companies for measurement engineering, offers a broad array of innovative analytical hplc columns. Profiling capsaicinoids in spicy foods and food products using the agilent 1290 infinity lc system and agilent zorbax poroshell 120 2. Agilent zorbax extend c18 column specifications and options zorbax extend c18 agilent zorbax extend c18 offers high efficiency and long life at high ph up to 11. Zorbax sb columns are designed to offer improved life time over traditional zorbax columns. If you are not completely satisfied with kinetex coreshell columns, send in your comparative data to a similar product within 45 days and keep the column for free. Reprosil 70 c18 equisil ods hypersil ods zorbax sb c18 novapak c18 supelcosil lcdb c18 ymc odsaq reprosilpur c18 aq symmetry c8 reprosil c8ab kromasil c8 zorbax. Syncronis c18 hplc columns consistent, predictable separations, column after column, time after time outstanding reproducibility highly pure, high surface area silica high carbon load for increased retention double endcapped for extra surface coverage highly inert towards basic compounds rigorously tested to ensure quality. Analyticsshop offers the whole range of agilent hplc columns material. Sb c18 and diisopropylsilanes sb c8, sb phenyl, sb c3, and sb cn are less susceptible to loss of the bonded phase by acid hydrolysis than traditional dimethylsilanes providing high column lifetime under these harsh conditions.
Agilent g67b 1200 series high performance autosampler. C18, c8, c3, and diphenyl functionalities c18 bonding a stericallyprotected c18 functionality, diisobutyl noctadecylsilane, to the zorbax. Thus, separations on sb aq are reproducible from run to. Widepore cyano selectvity for polar and nonpolar compounds. Spherical silica, monomerically bonded, endcapped, 100 a pore size. Symmetry c18 column under identical mobile phase conditions. Qualitybydesignbased method development using an agilent 1290 infinity ii lc.
The result is vastly improved column life and extraordinary chemical and temperature stability in the ph 16 range for a wide variety of phases sb cn, sb c8, and sb c18. Sb are advertised to be providing alternative selectivities one from another so i wouldnt expect identical results. Poroshell 120 sb c18 and poroshell 120 sb c8 nonendcapped for greater alternate selectivity. View online or download agilent zorbax 300sb c18 user manual. Zorbax rapid resolution ht high throughput columns up to 93% decrease in analysis time great resolution extended ph range, ph 1 to 9 a better way to faster separations. It is particularly wellsuited for use with aggressive mobile phases e. Agilent zorbax rapid resolution high definition sb c18 threaded column data sheet etary, highpressure, slurryloading technique to give optimim column efficiency. The best conditions for cmps in screening phase experiments. The uniform, spherical, eclipse plus c18 particles are based on an improved zorbax rxsil support that has a nominal surface area of 160 m. Agilent hplc column selection guide hplc columns in india. Zorbax reversedphase hplc column selection flow chart column. Agilent zorbax extendc18 column specifications and options.